Effect of Ethanolic Extract of Spondias pinnata on IschemiaReperfusion Injury and Ischemic Preconditioning of Heart (Only Abstract)

Abstract

Background and Purpose: Cardiac ischemic preconditioning represents the most powerful endogenous protective mechanism against ischemia. Spondias pinnata is rich source of flavonoids and phenolic compounds. The purpose of present study is to investigate the effect of ethanolic extract of stem bark of Spondias pinnata (100 mg/kg or 200 mg/kg) on ischemia- reperfusion injury and ischemic preconditioning of heart. Experimental Approach: Hearts from albino rats of Wistar strain were isolated and immediately mounted on Langendorff’s apparatus for retrograde perfusion. Myocardial ischemia reperfusion injury was produced by mounting isolated rat hearts on Langendorff’s apparatus and global ischemia was produced for 30 min followed by reperfusion for 120 min. In ischemic preconditioning group, the hearts were subjected to four episodes of 5 min ischemia and 5 min of reperfusion after 10 minutes of stabilization and then 30 min global ischemia, followed by 120 min of reperfusion. Myocardial infarct size was estimated macroscopically using TTC staining. The magnitude of cardiac injury was measured by Lactate Dehydrogenase (LDH) and Creatine Kinase (CK) concentration in the coronary effluent. The increase in infarct size and the release of LDH and CK are documented to be an index of I/R induced myocardial injury. Key Results: Spondias pinnata ethanolic extract significantly reduced ischemia reperfusioninduced myocardial injury in vitro, but it does not enhanced the cardioprotective effect of ischemic preconditioning. Conclusion and Implications: Administration of Spondias pinnata ethanolic extract may prevent ischemic and reperfusion induced myocardial injury probably by its antioxidant activity.